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Addgene inc d pei
D Pei, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d+pei/pMXs-MB+(Plasmid+%2331703)/pm35106457-276-3-9
Average 90 stars, based on 1 article reviews

d pei - by Bioz Stars, 2026-10

90/100 stars

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Transgenic Assay:

Article Title: Chromatin Accessibility Dynamics during iPSC Reprogramming.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-c-JUN (1:2000) Cell Signaling Technology Cat#9165; RRID: AB_2130165 HRP antibody anti-GAPDH (1:4000) KangChen Bio-tech Cat#KC-5G5; RRID: AB_2631280 Rabbit polyclonal anti-H3K27ac Abcam Cat#ab4729; RRID: AB_2118291 Rabbit polyclonal anti-mSIN3A Abcam Cat#ab3479; RRID: AB_303839 Mouse monoclonal anti-FLAG Sigma Cat#m1804; RRID: AB_2637089 Rabbit polyclonal anti-IgG Abcam Cat#ab37415; RRID: AB_2631996 Chemicals, Peptides, and Recombinant Proteins Leukemia Inhibitory Factor (LIF) Millipore Cat#ESGE107 CHIR99021 Sigma Cat#SML1046-5MG PD0325901 Sigma Cat#PZ0162-5MG FBS Moregate Batch#67301120 DMEM-Dulbecco’s Modified Eagle Medium, High Glucose Hyclone Cat#SH30022-2B GlutaMAX GIBCO Cat#35050079 Non-Essential Amino Acids Solution GIBCO Cat#11140076 2-Mercaptoethoethanol GIBCO Cat#21985-023 Sodium Pyruvate Solution GIBCO Cat#11360070 Penicillin-Streptomycin Solution Hyclone Cat#SV30010 Phosphate-Buffer Saline (PBS) GIBCO Cat#C14190500BT Trypsin-EDTA (0.25%), phenol red GIBCO Cat#25200114 0.1% Gelatin Solution Millipore Cat#ES-006-B TRI Reagent MRC Cat#TR118-200 Puromycin Dihydrochloride Thermo Fisher Scientific Cat#A1113803 Oligo-dT TaKaRa Cat#D511 RRI TaKaRa Cat#D2313A SsoAdvanced Universal SYBR Green Supermix Bio-Rad Cat#172-5274 Lipofectamine 3000 Reagent Thermo Fisher Cat#L3000001 IGEPAL CA-630 Sigma Cat#198596 DNase/RNase Free Deionized Water TIANGEN Cat#RT121 Critical Commercial Assays Nextera DNA library preparation kit Illumina Cat#FC-121-1031 QIAGEN MinElute kit QIAGEN Cat#28006 KAPA Library Quantification kit KAPA BIOSYSTEMS Cat#KK4824 TruSeq RNA Sample Prep kit Illumina Cat#RS-122-2001 VAHTS Turbo DNA Library Prep kit Vazyme Biotech Cat#TD-503 NextSeq500 High output 150 cycles Illumina Cat#FC-404-2002 NextSeq500 Mid output 150 cycles Illumina Cat#FC-404-2001 VAHTSTM Library Quantification Kit Vazyme Biotech Cat# NQ101 - NQ106 AMPure XP beads BEECKMAN COULTER Cat#A63882 Deposited Data ATAC-seq data, ChIP-seq data, RNA-seq data This paper GEO:GSE93029 (Continued on next page) e1 Cell Stem Cell 21, 819–833.e1–e6, December 7, 2017 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Cell Lines OG2 Mouse Embryonic Fibroblasts:CBA/ CaJ x C57BL/6J This paper NA OG2 Mouse Embryonic Stem cells:CBA/ CaJ x C57BL/6J This paper NA Platinum-E (Plat-E) A gift from The Fourth Military Medical University N/A HEK293T ATCC Cat#CRL-1126 Experimental Models: Organisms/Strains OG2 transgenic mice:CBA/CaJ x C57BL/6J The Jackson Laboratory Mouse strain datasheet: 004654 Oligonucleotides Oligonucleotides are summarized in Tables S4 and S5 N/A N/A Recombinant DNA pMX-Oct3/4 (Takahashi and Yamanaka, 2006) Addgene Cat#13366 pMX-Sox2 (Takahashi and Yamanaka, 2006) Addgene Cat#13367 pMX-Klf4 (Takahashi and Yamanaka, 2006) Addgene Cat#13370 pMX-DsRed Laboratory of D. Pei (Liu et al., 2015b) N/A pMX-c-Jun Laboratory of D. Pei (Liu et al., 2015b) N/A pMX-c-Fos Laboratory of D. Pei (Liu et al., 2015b) N/A pMX-Fosl1 Laboratory of D. Pei (Liu et al., 2015b) N/A pMX-Ets1 This paper N/A pMX-Elf3 This paper N/A pMX-Tead3 This paper N/A pMX-Mst1 This paper N/A pMX-Runx1 This paper N/A pMX-Runx2 This paper N/A pMX-Mef2c This paper N/A pMX-Sin3a This paper N/A pMX-Sin3b This paper N/A pMX-Rbbp7 This paper N/A pMX-Rbbp4 This paper N/A pMX-Sap18 This paper N/A pMX-Sap30 This paper N/A pMX-Hdac1 This paper N/A pMX-Hdac2 This paper N/A M69 pCMV pGL3 Luciferase was a gift from Randall Moon Addgene Cat#17186 Software and Algorithms EDAseq (Risso et al., 2011) https://bioconductor.org/packages/devel/ bioc/html/EDASeq.html Macs14 v1.4.2 (Zhang et al., 2008) http://liulab.dfci.harvard.edu/MACS/ 00README.html Dfilter v1.5 (Kumar et al., 2013) http://collaborations.gis.a-star.edu.sg/ cmb6/kumarv1/dfilter/tutorial.html Bowtie v2 (Langmead and Salzberg, 2012) http://bowtie-bio.sourceforge.net/bowtie2/ index.shtml (Continued on next page) Cell Stem Cell 21, 819–833.e1–e6, December 7, 2017 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rsem v1.2.22 (Li and Dewey, 2011) https://deweylab.github.io/RSEM/ Glbase (Hutchins et al., 2014) https://bitbucket.org/oaxiom/glbase/wiki/Home FASTQC Babraham Bioinformatics https://www.bioinformatics.babraham.ac.uk/ projects/fastqc/ Homer (Heinz et al., 2010) http://homer.ucsd.edu/homer/motif/ Bedtools Bedtools http://bedtools.readthedocs.io/en/latest/ deeptools (Ramı́rez et al., 2014) https://deeptools.readthedocs.io/en/latest/ Samtools Samtools http://www.htslib.org/ Goseq (Young et al., 2010) https://bioconductor.org/packages/release/ bioc/html/goseq.html DAVID (Huang et al., 2009) https://david.ncifcrf.gov/ Flowjo FLOWJO LLC https://www.flowjo.com/ GraphPad Prism 6.0 GraphPad Software https://www.graphpad.com/scientific- software/prism/ Illustrator Adobe System Software Ireland http://www.adobe.com/cn/products/cs6/ illustrator.html Photoshop Adobe System Software Ireland http://www.adobe.com/cn/products/cs6/ photoshop.html Bio-RAD CFX Manager BIO-RAD http://www.bio-rad.com/en-us/product/ cfx-manager-software?tab=Download ZEN Zeiss https://www.zeiss.com/microscopy/int/ software-cameras.html Accuri C6 Plus BD biosciences http://www.bdbiosciences.com/us/instruments/ research/cell-analyzers/bd-accuri/m/1294932/overview BD FACSAria II BD biosciences https://www.bdbiosciences.com/cn/home Dual-Luciferase Reporter Assay System Promega https://www.promega.com/products/reporter- assays-and-transfection/reporter-assays/dual_ luciferase-reporter-assay-system/?catNum=E1910 AB Applied Biosystems Gene Company Limited http://www.genehk.com/

Recombinant:

Luciferase:

Software:

Plasmid Preparation:

Article Title: Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency
Article Snippet: pMXs-ms-Klf5 , Laboratory of S. Yamanaka ( ) , Addgene plasmid Cat#50787. .. pMXs-MC , Laboratory of D. Pei ( ) , Addgene plasmid Cat#31704. .. pMXs-MB , Laboratory of D. Pei ( ) , Addgene plasmid Cat#31703.

Article Title: Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency
Article Snippet: pMXs-MC , Laboratory of D. Pei ( ) , Addgene plasmid Cat#31704. .. pMXs-MB , Laboratory of D. Pei ( ) , Addgene plasmid Cat#31703. .. pMXs-hNANOG (Plath) , Laboratory of K. Plath ( ) , Addgene plasmid Cat#18115.

Article Title: Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency.
Article Snippet: Crystal Data (KLF4 zinc finger) www.rcsb.org PDB ID: 2WBU Crystal Data (Klf4 zinc finger) www.rcsb.org PDB ID: 4M9E Raw and analyzed data of ChIP-seq This paper GEO: GSE157762 Raw and analyzed data of RNA-seq This paper GEO: GSE184449 Experimental models: Cell lines Mouse embryonic fibroblasts isolated from Tg(Nanog-GFP,Puro)1Yam mice featuring transgenic GFP driven by Nanog promoter This study NA Platinum-E (Plat-E) Retroviral Packaging Cell Line Cell Biolabs, Inc. Cat#RV-101 Platinum-GP (Plat-GP) Retroviral Packaging Cell Line Cell Biolabs, Inc. Cat#RV-103 Normal human skin fibroblast (NB1RGB) RIKEN BRC Cell Bank Cat#RCB0222 SL10 feeder cells Reprocell Cat#RCHEFC001 Experimental models: Organisms/strains Mouse: STOCK Tg(Nanog-GFP,Puro)1Yam RIKEN BRC (Okita et al., 2007) RBRC 02290 Oligonucleotides Primers for plasmid construction, see Table S2 (KLF4 alanine mutants) This paper Recombinant DNA Plasmids made in this study, see Table S3 This paper pMXs-hKLF4 Laboratory of Plath (Lowry et al., 2008) Addgene plasmid Cat#17967 pMXs-hOCT3/4 Laboratory of S. Yamanaka (Takahashi et al., 2007) Addgene plasmid Cat#17217 pMXs-hSOX2 Laboratory of S. Yamanaka (Takahashi et al., 2007) Addgene plasmid Cat#17218 pMXs-Hu-L-Myc Laboratory of S. Yamanaka (Nakagawa et al., 2008) Addgene plasmid #26022 pMXs-mRFP1 Laboratory of J. Ellis (Hotta et al., 2009) Addgene plasmid Cat#21315 pMXs-p53DD Laboratory of S. Yamanaka (Hong et al., 2009) Addgene plasmid Cat#22729 pCMV-VSV-G Laboratory of H. Miyoshi, RIKEN BRC DNA Bank Cat#RDB04392 pMXs-hFOXH1 Laboratory of S. Yamanaka (Takahashi et al., 2014) Addgene plasmid Cat#59183 pMXs-GLIS1 Laboratory of S. Yamanaka (Maekawa et al., 2011; Worringer et al., 2014) Addgene plasmid Cat#30166 pMXs-ms-Klf5 Laboratory of S. Yamanaka (Nakagawa et al., 2008) Addgene plasmid Cat#50787 (Continued on next page) iScience 25, 103525, January 21, 2022 21 .. pMXs-MC Laboratory of D. Pei (Liao et al., 2011) Addgene plasmid Cat31704 pMXs-MB Laboratory of D. Pei (Liao et al., 2011) Addgene plasmid Cat#31703 pMXs-hNANOG (Plath) Laboratory of K. Plath (Lowry et al., 2008) Addgene plasmid Cat#18115 SeVdp(fKgOSMaB) This paper NA SeVdp(fK[507]gOSMaB) This paper NA .. index.shtml MACS2 (Zhang et al., 2008) https://github.com/taoliu/MACS CLC genomics workbench Qiagen https://digitalinsights.qiagen.com/ ja/qiagen-clc-genomics-workbench/

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